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Immunocytochemistry is a special case of histochemistry that uses selective antibodies against a variety of chemical epitopes of the nervous system to selectively stain particular cell types, axonal fascicles, neuropiles, glial processes or blood vessels, or specific intracytoplasmic or intranuclear proteins and other immunogenetic molecules, e.g., neurotransmitters.

Immunoreacted transcription factor proteins reveal genomic readout in terms of translated protein.

One advantage of working with this worm is that the nervous system of the hermaphrodite contains exactly 302 neurons, always in the same places, making identical synaptic connections in every worm.

Brenner's team sliced worms into thousands of ultrathin sections and photographed every section under an electron microscope, then visually matched fibers from section to section, to map out every neuron and synapse in the entire body, to give a complete connectome of the nematode.

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A mid-sagittal plane divides the body and brain into left and right halves; sagittal sections in general are parallel to this median plane, moving along the medial-lateral dimension(see the image above).

The term sagittal refers etymologically to the median suture between the right and left parietal bones of the cranium, known classically as sagittal suture, because it looks roughly like an arrow by its confluence with other sutures (sagitta; arrow in Latin).

This immensely increases the capacity of researchers to distinguish between different cell types (such as neurons and glia) in various regions of the nervous system.

The brain is small and simple in some species, such as the nematode worm, where the body plan is quite simple: a tube with a hollow gut cavity running from the mouth to the anus, and a nerve cord with an enlargement (a ganglion) for each body segment, with an especially large ganglion at the front, called the brain.

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In contrast to animals with radial symmetry, whose nervous system consists of a distributed network of cells, animals with bilateral symmetry have segregated, defined nervous systems, and thus we can make much more precise statements about their neuroanatomy.

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